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384 = 96 x 4, isn’t it? - Save time with 384-well formats
실험실 아카데미
- 세포생물학
- 피펫팅 및 분주
- 효율성
- 피펫
- 피펫 팁
- 논문
It cannot be ignored: The workload in labs is constantly growing. 25 years ago, working with 96-well plates may have been progressive but nowadays an increasing number of labs are changing to 384-well plate format. Super, now you have four 96-well plates on a single plate – just with wells in miniature format. But what is the advantage? At first glance, there seems to be only disadvantages –time to take a closer look at this system.
Save time by using less material?
Instead of storing four 96-well plates, you only need room for one plate. That looks especially good in the fridge, the freezer or the store cupboard. But it gets even better: One tip box or rack contains 4 times more tips than the standard (96) format - saving you a lot of space on the bench. This makes two real plus points! Less material also means that you can organize your workspace more efficiently and therefore save time.
In cases where it is possible to run an analysis in e.g. 40 µL instead of 150 µL, the 384-well plates provide many advantages. First and foremost, you save reagent. For example: All four 96-well plate filled with 120 µL per well needs a total volume of approximately 46 mL. If the same analysis is miniaturized to a reaction volume of 40 µL per well, the total volume is decreased to 15 mL.
Expensive reagents such as those used in molecular biology can be saved and the cost per analysis reduced. Of course, this is only feasible if the reaction or analysis can be miniaturized.
Are you able to fill a 384-well plate manually within a minute?
Another advantage is you save time. Are you able to fill a 384-well plate manually within a minute? It is possible to fill 384-well plates with 8/12-channel pipettes. But then only every second well can be filled at one time.
On the one hand, this is highly error-prone ("oh, my centrifuge is ready… which was the next well to be filled?"). On the other hand, it is tiring and time-consuming. Just switching the plates to 384-well format and filling them with 8/12-channel pipettes is false economy.
What you need is a reliable 16 or 24-channel pipette whose cone distances are harmonized to the distance between wells of a 384-well plate. And suddenly the work becomes more efficient.
Instead of storing four 96-well plates, you only need room for one plate. That looks especially good in the fridge, the freezer or the store cupboard. But it gets even better: One tip box or rack contains 4 times more tips than the standard (96) format - saving you a lot of space on the bench. This makes two real plus points! Less material also means that you can organize your workspace more efficiently and therefore save time.
In cases where it is possible to run an analysis in e.g. 40 µL instead of 150 µL, the 384-well plates provide many advantages. First and foremost, you save reagent. For example: All four 96-well plate filled with 120 µL per well needs a total volume of approximately 46 mL. If the same analysis is miniaturized to a reaction volume of 40 µL per well, the total volume is decreased to 15 mL.
Expensive reagents such as those used in molecular biology can be saved and the cost per analysis reduced. Of course, this is only feasible if the reaction or analysis can be miniaturized.
Are you able to fill a 384-well plate manually within a minute?
Another advantage is you save time. Are you able to fill a 384-well plate manually within a minute? It is possible to fill 384-well plates with 8/12-channel pipettes. But then only every second well can be filled at one time.
On the one hand, this is highly error-prone ("oh, my centrifuge is ready… which was the next well to be filled?"). On the other hand, it is tiring and time-consuming. Just switching the plates to 384-well format and filling them with 8/12-channel pipettes is false economy.
What you need is a reliable 16 or 24-channel pipette whose cone distances are harmonized to the distance between wells of a 384-well plate. And suddenly the work becomes more efficient.
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3 x faster than alternate well pipetting
Once you are holding the optimal tool, you can get started. How much time do you think can be saved with a 24-channel pipette compared to a 12-channel? Lab personnel that had the chance to apply such an innovative pipette/pipette tip system reported: "3 times as fast as with 12-channel" and "50 % time saving" as well as "we have saved a complete working day". As an additional side-effect, incubations on a plate do not need to be started staggered or row-wise. You can now fill a plate in less than a minute.
Find more information about the new Eppendorf Research plus and Xplorer plus 16/24-channel pipettes and pipette tips epT.I.P.S.® 384 here.
Once you are holding the optimal tool, you can get started. How much time do you think can be saved with a 24-channel pipette compared to a 12-channel? Lab personnel that had the chance to apply such an innovative pipette/pipette tip system reported: "3 times as fast as with 12-channel" and "50 % time saving" as well as "we have saved a complete working day". As an additional side-effect, incubations on a plate do not need to be started staggered or row-wise. You can now fill a plate in less than a minute.
Find more information about the new Eppendorf Research plus and Xplorer plus 16/24-channel pipettes and pipette tips epT.I.P.S.® 384 here.
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